The terminator mouse: salvation for primary cell culture
نویسندگان
چکیده
منابع مشابه
Mouse liver cell culture. II. Primary culture.
Mouse hepatocytes in primary culture were characterized. Hepatocytes were isolated by the two-step hepatic portal vein perfusion method described previously. An optimal cell attachment of 43% was noted after 2 h incubation in 10% fetal bovine serum. Minimal attachment (less than 7%) occurred in serumless medium. Serum concentrations above 10% and attachment durations greater that 2 h resulted i...
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ژورنال
عنوان ژورنال: Kidney International
سال: 2013
ISSN: 0085-2538
DOI: 10.1038/ki.2013.288